Displaying and Using UV Spectra
Displaying and Overlaying Single Spectra
In addition to peak spectra (= spectrum in the peak maximum or spectrum at a defined peak height), Chromeleon is capable of displaying any other spectrum of a chromatogram at the time t.
To extract any spectra of a chromatogram via a mouse click, follow the steps below:
Enable the Spectra/I-t Plots Tool on the context menu or click the corresponding icon on the Integration Toolbar
A spectra symbol that is appended to the mouse pointer indicates that the mode was changed.
Click anywhere in the chromatogram to display the corresponding spectrum.
Repeat the action while pressing the SHIFT key to overlay several spectra.
Displaying Spectra of Different Samples
To compare spectra of different samples objectively, perform a chromatogram comparison:
Compare two chromatograms by displaying an additional chromatogram in the Integration window (see Integration Chromatogram Comparison).
Enable the Spectra/I-t Plots Tool and select single spectra by clicking various places in the chromatogram while pressing the SHIFT key.
Note:
UV spectra are overlaid in one window.